Modifying Enzymes
New England Biolabs, Inc. Micrococcal Nuclease – 320000 gel units
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate. Both DNA and RNA are degraded to 3 phosphomononucleotides and dinucleotides. Micrococcal Nuclease works well with the SimpleChIP Enzymatic Chromatin IP Kit (Agarose Beads) from Cell Signaling Technology.
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase (Sialidase) from Vibrio cholerae
Neuraminidase is an acylneuraminyl hydrolase which hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Biotang Inc Carbonic Anhydrase II E.coli Recombinant, Carbonic Anhydrase II E.coli, Synonyms Carbonic anhydrase 2, Carbonate dehydratase 2, can, cynT2, yadF, b0126, JW0122, Carbonic Anhydrase II.
Carbonic Anhydrase II E.coli Recombinant, Carbonic Anhydrase II E.coli, Synonyms Carbonic anhydrase 2, Carbonate dehydratase 2, can, cynT2, yadF, b0126, JW0122, Carbonic Anhydrase II.
Worthington Biochemical Corporation Protease S. aureus Sequencing Grade
Protease S. aureus Sequencing Grade
Worthington Biochemical Corporation 1G PEPSIN A
Pepsin A,1 gm,Source:Porcine Stomach,Minimum Activity:=2500 units per mg dry weight,2X crystallized from dilute alcohol. A lyophilized powder. Store at 2-8degreeC,Unit Definition:1 unit releases 0.001 A280 as TCA soluble hydrolysis products
Roche Diagnostics PROTEASE 1
Protease 1, Component: Proprietary ingredients, 45 to 55%, Sodium azide, CAS No.: 26628-22-8, 0.1%, Appearance: Clear, Colorless liquid, Odorless, Solubility: Miscible in water, pH: neutral, Stability: stable
Sigma Aldrich Fine Chemicals Biosciences Malonyl 13C3 coenzyme A li10MG
Malonyl 13C3 coenzyme A li10MG
Sigma Aldrich Fine Chemicals Biosciences T4 DNA Ligase recombinant
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3-hydroxyl- and 5-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.ContentsT4 DNA Ligase supplied with 10x concentrated ligation buffer that includes ATP.
Worthington Biochemical Corporation CHYMOTRYPSIN ALPHA TLCK 100MG
Chymotrypsin, alpha, TLCK treated, Storage: 2degree to 8degreeC, Source: Bovine pancreas, Pack Size: 100mg.
STEMCELL Technologies Proteinase K Solution, Size: 4 mL
Proteinase K is a serine protease that exhibits broad cleavage activity, cutting peptide bonds adjacent to the carboxylic group in aliphatic and aromatic amino acids. Proteinase K is used for a variety of applications, including removal of nucleases from DNA and RNA samples and terminating enzymatic reactions. Proteinase K Solution is in a ready-to-use format that is stable at room temperature and active over a pH range of 4.3 - 12.0 in 0.5% SDS or 1% Triton X-100. Product is free of nuclease activity. Proteinase K Solution is required for use with ArciTect™ T7 Endonuclease I Kit (Catalog #76021).
Roche Diagnostics General Chemistry Gamma Glutamyl Transferase (GGT) For Cobas Integra Analyzer
General Chemistry Gamma Glutamyl Transferase (GGT) For Cobas Integra Analyzer.